Nepali Congress leader and Minister for Drinking Water, Energy and Irrigation Shyam Bahadur Khadka of the Bagmati Province government tendered his resignation on Tuesday.
Earlier, four ministers had resigned for their post.
Minister for Youth and Sports Min Krishna Maharjan, Minister Internal Affairs and Law Suraj Chandra Lamichhane, Minister for Culture and Tourism Bimal Thakuri, and Minister for Cooperatives and Poverty Alleviation Madhu Kumar Shrestha had resigned.
After submitting their resignations, they filed a no-confidence motion against Chief Minister Bahadur Singh Lama to remove him as the Nepali Congress provincial Parliamentary Party leader.
Along with Khadka, 22 lawmakers have signed the proposal.
After resigning from the post of minister, he signed the no-confidence motion against Lama.
The Bagmati Province government has 14 ministries, including the Chief Minister.
Of these, ruling coalition party CPN-UML has six ministers and Congress has eight.