Minister for Culture, Tourism and Civil Aviation Prem Ale resigned from his post on Sunday.
He handed over his resignation letter to Chairman Madhav Nepal in the party office, Chairman Nepal's Secretariat said.
Withdrawing his signature from the group 10 CPN (Unified Socialist) lawmakers who had submitted a memorandum expressing dissatisfaction over the party's decision to reshuffle ministers on Friday, Ale said that he would resign within 24 hours.
Ale, who handed over his resignation letter to Chairman Nepal, is preparing to organize a press conference.
Earlier on June 5, the Secretariat meeting of the CPN (Unified Socialist) had decided to recall the ministers representing the party in the government.
But, Prime Minister Sher Bahadur Deuba and CPN (Maoist Centre) Chairman Pushpa Kamal Dahal said that the CPN (US)'s decision to reshuffle the ministers will be implemented after the completion of budget discussions.
A Central Committee meeting held on Saturday had decided that the party would not wait for Prime Minister Deuba and Dahal now and would take the delay as an insult.